1
/
of
1
ELK Biotechnology
SKU:ELK5497
Mouse OB(Obestatin) ELISA Kit
Mouse OB(Obestatin) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: Obestatin
Assay Type: Competitive Inhibition
Sensitivity: 26.8 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Metabolic pathway;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse OB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OB in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 26.8 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Metabolic pathway;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse OB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OB in the samples is then determined by comparing the OD of the samples to the standard curve.
Share
