Human NKX3-1(NK3 Homeobox Protein 1) ELISA Kit

Alternative Names: BAPX2; NKX3; NKX3.1; NKX3A; NK3 transcription factor related, locus 1; Homeobox protein NK-3 homolog A
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX3-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX3-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX3-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX3-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q99801