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ELK Biotechnology
SKU:ELK5382
Mouse ACV-A(Activin A) ELISA Kit
Mouse ACV-A(Activin A) ELISA Kit
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Alternative Names: ACV-A; Activin Beta A Beta A Homodimer
Assay Type: Sandwich
Sensitivity: 5 pg/mL
standard: 600 pg/mL
Detection range: 9.38-600 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACVA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACVA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACVA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q04998
Assay Type: Sandwich
Sensitivity: 5 pg/mL
standard: 600 pg/mL
Detection range: 9.38-600 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACVA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACVA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACVA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q04998
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