Human FOLR2(Folate Receptor 2, Fetal) ELISA Kit

Alternative Names: FBP/PL-1; FR-P3; FR-beta; beta-hFR; Folate receptor, fetal/placental; Placental folate-binding protein
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Reproductive science;Genetic science;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FOLR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FOLR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FOLR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FOLR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P14207