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ELK Biotechnology
SKU:ELK5242
Human PTGR1(Prostaglandin Reductase 1) ELISA Kit
Human PTGR1(Prostaglandin Reductase 1) ELISA Kit
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Alternative Names: LTB4DH; ZADH3; NADP-dependent leukotriene B4 12-hydroxydehydrogenase; Zinc Binding Alcohol Dehydrogenase Domain Containing 3; 15-oxoprostaglandin 13-reductase
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTGR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q14914
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTGR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q14914
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