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ELK Biotechnology
SKU:ELK5229
Human COX1(Cytochrome C Oxidase Subunit I) ELISA Kit
Human COX1(Cytochrome C Oxidase Subunit I) ELISA Kit
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Alternative Names: MT-CO1; MTCO1; COI; COXI; Mitochondrially Encoded Cytochrome C Oxidase I; Cytochrome c oxidase polypeptide I
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COX1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P00395
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COX1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P00395
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