Mouse TGFb2(Transforming Growth Factor Beta 2) ELISA Kit

Alternative Names: TGF-B2; TGF-Beta2; G-TSF;  LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor
Assay Type: Competitive Inhibition
Sensitivity: 8.95 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Assay length: 2.5h
Research Area: Cytokine;Infection immunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TGFb2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P27090