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ELK Biotechnology
SKU:ELK5213
Human MFN1(Mitofusin 1) ELISA Kit
Human MFN1(Mitofusin 1) ELISA Kit
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Alternative Names: Hfzo1; Hfzo2; Transmembrane GTPase MFN1; Fzo homolog
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MFN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MFN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MFN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MFN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8IWA4
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MFN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MFN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MFN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MFN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8IWA4
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