1
/
of
1
ELK Biotechnology
SKU:ELK5122
Mouse Btk(Bruton'S Tyrosine Kinase) ELISA Kit
Mouse Btk(Bruton'S Tyrosine Kinase) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: AGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA; Bruton Agammaglobulinemia Tyrosine Kinase; B-cell progenitor kinase
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Infection immunity;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Btk. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Btk. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Btk, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Btk in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P35991
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Infection immunity;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Btk. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Btk. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Btk, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Btk in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P35991
Share
