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ELK Biotechnology
SKU:ELK4865
Human PLOD2(Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 2) ELISA Kit
Human PLOD2(Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 2) ELISA Kit
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Alternative Names: TLH; LH2; Telopeptide Lysyl Hydroxylase; Lysyl hydroxylase 2
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLOD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLOD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLOD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLOD2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O00469
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLOD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLOD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLOD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLOD2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O00469
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