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ELK Biotechnology
SKU:ELK4833
Rat MAOA(Monoamine Oxidase A) ELISA Kit
Rat MAOA(Monoamine Oxidase A) ELISA Kit
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Alternative Names: MAO-A; Amine oxidase [flavin-containing] A
Assay Type: Sandwich
Sensitivity: 55 pg/mL
standard: 8000 pg/mL
Detection range: 125-8000 pg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Neuro science;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAOA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAOA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P21396
Assay Type: Sandwich
Sensitivity: 55 pg/mL
standard: 8000 pg/mL
Detection range: 125-8000 pg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Neuro science;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAOA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAOA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P21396
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