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ELK Biotechnology
SKU:ELK4610
Mouse aCTx(Alpha-Crosslaps) ELISA Kit
Mouse aCTx(Alpha-Crosslaps) ELISA Kit
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$558.00 USD
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Alternative Names: Alpha CTx; a-CTXI; aCTX-I; A-Cr; Type I Collagen C-Telopeptide-Related Fraction
Assay Type: Competitive Inhibition
Sensitivity: 26.57 pg/mL
standard: 6000 pg/mL
Detection range: 93.75-6000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 2.5h
Research Area: Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse aCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse aCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse aCTx in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 26.57 pg/mL
standard: 6000 pg/mL
Detection range: 93.75-6000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 2.5h
Research Area: Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse aCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse aCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse aCTx in the samples is then determined by comparing the OD of the samples to the standard curve.
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