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ELK Biotechnology
SKU:ELK4588
Human RIPK1(Receptor Interacting Serine Threonine Kinase 1) ELISA Kit
Human RIPK1(Receptor Interacting Serine Threonine Kinase 1) ELISA Kit
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Alternative Names: RIP; Receptor-Interacting Protein; Cell death protein RIP; Serine/threonine-protein kinase RIP
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RIPK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RIPK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RIPK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RIPK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q13546
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RIPK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RIPK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RIPK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RIPK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q13546
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