1
/
of
1
ELK Biotechnology
SKU:ELK4460
Mouse TOP2(Topoisomerase II) ELISA Kit
Mouse TOP2(Topoisomerase II) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme
Assay Type: Sandwich
Sensitivity: 0.112 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TOP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TOP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TOP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TOP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q01320
Assay Type: Sandwich
Sensitivity: 0.112 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TOP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TOP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TOP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TOP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q01320
Share
