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ELK Biotechnology
SKU:ELK4391
Human Tb4(Thymosin Beta 4) ELISA Kit
Human Tb4(Thymosin Beta 4) ELISA Kit
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Alternative Names: TMSB4X; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide
Assay Type: Competitive Inhibition
Sensitivity: 0.88 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Metabolic pathway;Endocrinology;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Tb4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tb4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tb4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P62328
Assay Type: Competitive Inhibition
Sensitivity: 0.88 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Metabolic pathway;Endocrinology;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Tb4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tb4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tb4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P62328
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