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ELK Biotechnology
SKU:ELK4197
Human TMEM27(Transmembrane Protein 27) ELISA Kit
Human TMEM27(Transmembrane Protein 27) ELISA Kit
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Alternative Names: NX17; Collectrin
Assay Type: Sandwich
Sensitivity: 0.063 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TMEM27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMEM27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TMEM27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMEM27 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9HBJ8
Assay Type: Sandwich
Sensitivity: 0.063 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TMEM27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMEM27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TMEM27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMEM27 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9HBJ8
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