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ELK Biotechnology
SKU:ELK4126
Human CPA4(Carboxypeptidase A4) ELISA Kit
Human CPA4(Carboxypeptidase A4) ELISA Kit
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Alternative Names: Carboxypeptidase A3; Carboxypeptidase A4; CBPA4_HUMAN; CPA3 ; Cpa4
Assay Type: Sandwich
Sensitivity: 0.068 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CPA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CPA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CPA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CPA4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9UI42
Assay Type: Sandwich
Sensitivity: 0.068 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CPA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CPA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CPA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CPA4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9UI42
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