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ELK Biotechnology
SKU:ELK4057
Human KNS2(Kinesin 2) ELISA Kit
Human KNS2(Kinesin 2) ELISA Kit
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Alternative Names: KLC; KLC1; KNS2A; hKLC1S; hKLC1N; hKLC1P; hKLC1G; hKLC1R; hKLC1J; hKLC1B; Kinesin Light Chain 1
Assay Type: Sandwich
Sensitivity: 0.104 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KNS2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KNS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KNS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KNS2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O00139
Assay Type: Sandwich
Sensitivity: 0.104 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KNS2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KNS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KNS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KNS2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O00139
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