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ELK Biotechnology
SKU:ELK4018
Human LPHN3(Latrophilin 3) ELISA Kit
Human LPHN3(Latrophilin 3) ELISA Kit
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Alternative Names: CIRL3; LEC3; Calcium-independent alpha-latrotoxin receptor 3; Lectomedin-3
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LPHN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LPHN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LPHN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LPHN3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9HAR2
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LPHN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LPHN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LPHN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LPHN3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9HAR2
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