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ELK Biotechnology
SKU:ELK3994
Mouse TGM3(Transglutaminase 3, Epidermal) ELISA Kit
Mouse TGM3(Transglutaminase 3, Epidermal) ELISA Kit
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Alternative Names: TGE; Egt; Protein-Glutamine Gamma-Glutamyltransferase E; Transglutaminase E
Assay Type: Sandwich
Sensitivity: 0.133 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Infection immunity;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TGM3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGM3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TGM3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGM3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q08189
Assay Type: Sandwich
Sensitivity: 0.133 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Infection immunity;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TGM3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGM3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TGM3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGM3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q08189
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