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ELK Biotechnology
SKU:ELK3856
Human LRRK2(Leucine Rich Repeat Kinase 2) ELISA Kit
Human LRRK2(Leucine Rich Repeat Kinase 2) ELISA Kit
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Alternative Names: AURA17; PARK8; RIPK7; ROCO2; Dardarin ; Parkinson Disease(Autosomal Dominant)8; Leucine-rich repeat serine/threonine-protein kinase 2
Assay Type: Sandwich
Sensitivity: 32 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LRRK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LRRK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LRRK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LRRK2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q5S007
Assay Type: Sandwich
Sensitivity: 32 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LRRK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LRRK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LRRK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LRRK2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q5S007
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