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ELK Biotechnology
SKU:ELK3788
Human DUSP5(Dual Specificity Phosphatase 5) ELISA Kit
Human DUSP5(Dual Specificity Phosphatase 5) ELISA Kit
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Alternative Names: DUSP; HVH3; Dual specificity protein phosphatase hVH3
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DUSP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DUSP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DUSP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DUSP5 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q16690
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DUSP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DUSP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DUSP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DUSP5 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q16690
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