1
/
of
1
ELK Biotechnology
SKU:ELK3623
Human CST6(Cystatin 6) ELISA Kit
Human CST6(Cystatin 6) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: Cystatin E/M
Assay Type: Sandwich
Sensitivity: 0.067 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, sweat, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CST6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CST6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CST6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CST6 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q15828
Assay Type: Sandwich
Sensitivity: 0.067 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, sweat, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CST6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CST6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CST6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CST6 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q15828
Share
