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ELK Biotechnology
SKU:ELK3571
Human CAPS(Calcyphosine) ELISA Kit
Human CAPS(Calcyphosine) ELISA Kit
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Alternative Names: CAPS1; Thyroid Protein p24; Calcyphosin
Assay Type: Sandwich
Sensitivity: 0.05 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Immune molecule;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAPS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q13938
Assay Type: Sandwich
Sensitivity: 0.05 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Immune molecule;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAPS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q13938
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