Human MT1M(Metallothionein 1M) ELISA Kit

Alternative Names: MT1K;
Assay Type: Competitive Inhibition
Sensitivity: 0.96 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2.5h
Research Area: Tumor immunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MT1M protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MT1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MT1M in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8N339