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ELK Biotechnology
SKU:ELK3516
Human CDCP1(CUB Domain Containing Protein 1) ELISA Kit
Human CDCP1(CUB Domain Containing Protein 1) ELISA Kit
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Alternative Names: CD318; CDCP1; SIMA135; TRASK; Membrane glycoprotein gp140; Transmembrane and associated with src kinases; Subtractive immunization M plus HEp3-associated 135 kDa
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDCP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDCP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDCP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9H5V8
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDCP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDCP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDCP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9H5V8
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