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ELK Biotechnology
SKU:ELK3507
Human CAPZb(Capping Protein Muscle Z Line Beta) ELISA Kit
Human CAPZb(Capping Protein Muscle Z Line Beta) ELISA Kit
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Alternative Names: CAPZ; CAPB; CAPPB; F-Actin-Capping Protein Subunit Beta
Assay Type: Sandwich
Sensitivity: 0.06 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell Lysates and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAPZb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAPZb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAPZb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAPZb in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P47756
Assay Type: Sandwich
Sensitivity: 0.06 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell Lysates and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAPZb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAPZb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAPZb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAPZb in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P47756
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