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ELK Biotechnology
SKU:ELK3309
Human PZP(Pregnancy Zone Protein) ELISA Kit
Human PZP(Pregnancy Zone Protein) ELISA Kit
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Alternative Names: CPAMD6; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6
Assay Type: Sandwich
Sensitivity: 0.121 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PZP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PZP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PZP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PZP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P20742
Assay Type: Sandwich
Sensitivity: 0.121 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PZP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PZP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PZP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PZP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P20742
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