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ELK Biotechnology
SKU:ELK3181
Human OGDH(Oxoglutarate Dehydrogenase) ELISA Kit
Human OGDH(Oxoglutarate Dehydrogenase) ELISA Kit
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Alternative Names: AKGDH; E1k; OGDC; Alpha-Ketoglutarate Dehydrogenase,Lipoamide; 2-Oxoglutarate Dehydrogenase E1 Component,Mitochondrial
Assay Type: Sandwich
Sensitivity: 0.54 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OGDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OGDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OGDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OGDH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q02218
Assay Type: Sandwich
Sensitivity: 0.54 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OGDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OGDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OGDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OGDH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q02218
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