1
/
of
1
ELK Biotechnology
SKU:ELK3053
Human PSPN(Persephin) ELISA Kit
Human PSPN(Persephin) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: PSP
Assay Type: Sandwich
Sensitivity: 12.9 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Cytokine;Infection immunity;Neuro science;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSPN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O60542
Assay Type: Sandwich
Sensitivity: 12.9 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Cytokine;Infection immunity;Neuro science;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSPN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O60542
Share
