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ELK Biotechnology
SKU:ELK2998
Human S100A7(S100 Calcium Binding Protein A7) ELISA Kit
Human S100A7(S100 Calcium Binding Protein A7) ELISA Kit
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Alternative Names: S100-A7; PSOR1; S100A7c; Psoriasin 1
Assay Type: Sandwich
Sensitivity: 51 pg/mL
standard: 8000 pg/mL
Detection range: 125-8000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100A7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100A7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100A7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S100A7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P31151
Assay Type: Sandwich
Sensitivity: 51 pg/mL
standard: 8000 pg/mL
Detection range: 125-8000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100A7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100A7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100A7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S100A7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P31151
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