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ELK Biotechnology
SKU:ELK2943
Human PROM1(Prominin 1) ELISA Kit
Human PROM1(Prominin 1) ELISA Kit
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Alternative Names: CD133; PROML1; Macular Dystrophy,Retinal 2; Stargardt Disease 4(Autosomal Dominant); Prominin-like protein 1; Antigen AC133
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PROM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PROM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PROM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PROM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O43490
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PROM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PROM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PROM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PROM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O43490
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