Mouse PK2(Prokineticin 2) ELISA Kit

Alternative Names: PK-2; Bv8; MIT1; KAL4; Protein Bv8 Homolog
Assay Type: Competitive Inhibition
Sensitivity: 94.4 pg/mL
standard: 20000 pg/mL
Detection range: 312.5-20000 pg/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PK2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PK2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9QXU7