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ELK Biotechnology
SKU:ELK2800
Human CFI(Complement Factor I) ELISA Kit
Human CFI(Complement Factor I) ELISA Kit
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Alternative Names: CF-I; C3b-INA; FI; IF; KAF; Factor I; Konglutinogen-Activating Factor; C3b-Inactivator; C3B/C4B inactivator
Assay Type: Sandwich
Sensitivity: 0.072 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Immune molecule;Hematology;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFI in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P05156
Assay Type: Sandwich
Sensitivity: 0.072 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Immune molecule;Hematology;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFI in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P05156
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