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ELK Biotechnology
SKU:ELK2777
Human MBP(Myelin Basic Protein) ELISA Kit
Human MBP(Myelin Basic Protein) ELISA Kit
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Alternative Names: PRG2; BMPG; Proteoglycan 2; Bone Marrow,Natural Killer Cell Activator; Eosinophil Granule Major Basic Protein; Proteoglycan 2,Bone Marrow; Pregnancy-associated major basic
Assay Type: Sandwich
Sensitivity: 1.34 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Rheumatology;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MBP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02686
Assay Type: Sandwich
Sensitivity: 1.34 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Rheumatology;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MBP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02686
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