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ELK Biotechnology
SKU:ELK2702
Human IL17B(Interleukin 17B) ELISA Kit
Human IL17B(Interleukin 17B) ELISA Kit
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Alternative Names: IL-20; Cytokine Zcyto7; Neuronal interleukin-17-related factor
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Cytokine;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL17B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL17B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL17B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL17B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9UHF5
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Cytokine;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL17B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL17B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL17B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL17B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9UHF5
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