Human TRH(Thyrotropin Releasing Hormone) ELISA Kit

Alternative Names: TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
Assay Type: Competitive Inhibition
Sensitivity: 2.7 pg/mL
standard: 500 pg/mL
Detection range: 7.82-500 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;Neuro science;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P20396