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ELK Biotechnology
SKU:ELK2583
Rat PAI2(Plasminogen Activator Inhibitor 2) ELISA Kit
Rat PAI2(Plasminogen Activator Inhibitor 2) ELISA Kit
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Alternative Names: SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Plasma
Assay length: 3.5h
Research Area: Hematology;Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P29524
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Plasma
Assay length: 3.5h
Research Area: Hematology;Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P29524
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