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ELK Biotechnology
SKU:ELK2203
Human MUC7(Mucin 7, Secreted) ELISA Kit
Human MUC7(Mucin 7, Secreted) ELISA Kit
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Alternative Names: MG2; Mucin 7,Salivary; Apo-MG2; Salivary mucin-7
Assay Type: Sandwich
Sensitivity: 0.123 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, saliva, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUC7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUC7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUC7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUC7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8TAX7
Assay Type: Sandwich
Sensitivity: 0.123 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, saliva, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUC7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUC7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUC7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUC7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8TAX7
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