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ELK Biotechnology

SKU:ELK1946

Human dSIP(Delta-Sleep Inducing Peptide) ELISA Kit

Human dSIP(Delta-Sleep Inducing Peptide) ELISA Kit

Regular price $558.00 USD
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Alternative Names: DSIPI; TSC22D3; DIP; GILZ; TSC-22R; hDIP; TSC22 Domain Family Protein 3; Glucocorticoid-Induced Leucine Zipper; Delta Sleep Inducing Peptide,Immunoreactor
Assay Type: Competitive Inhibition
Sensitivity: 7.49 pg/mL
standard: 1500 pg/mL
Detection range: 23.44-1500 pg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 2.5h
Research Area: Reproductive science;Neuro science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human dSIP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dSIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dSIP in the samples is then determined by comparing the OD of the samples to the standard curve.
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