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ELK Biotechnology
SKU:ELK1909
Mouse BMP1(Bone Morphogenetic Protein 1) ELISA Kit
Mouse BMP1(Bone Morphogenetic Protein 1) ELISA Kit
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Alternative Names: PCOLC; PCP; TLD; mTld; Procollagen C Proteinase; Mammalian tolloid protein
Assay Type: Sandwich
Sensitivity: 29 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P98063
Assay Type: Sandwich
Sensitivity: 29 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P98063
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