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ELK Biotechnology
SKU:ELK1842
Human vWA3A(Von Willebrand Factor A Domain Containing Protein 3A) ELISA Kit
Human vWA3A(Von Willebrand Factor A Domain Containing Protein 3A) ELISA Kit
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Alternative Names: VWA3A
Assay Type: Sandwich
Sensitivity: 0.15 ng/mL
standard: 30 ng/mL
Detection range: 0.47-30 ng/mL
Sample type: Plasma
Assay length: 3.5h
Research Area: Developmental science;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vWA3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human vWA3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human vWA3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vWA3A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: A6NCI4
Assay Type: Sandwich
Sensitivity: 0.15 ng/mL
standard: 30 ng/mL
Detection range: 0.47-30 ng/mL
Sample type: Plasma
Assay length: 3.5h
Research Area: Developmental science;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vWA3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human vWA3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human vWA3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vWA3A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: A6NCI4
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