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ELK Biotechnology
SKU:ELK1829
Human AR(Androgen Receptor) ELISA Kit
Human AR(Androgen Receptor) ELISA Kit
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Alternative Names: AIS; DHTR; HUMARA; KD; NR3C4; SBMA; SMAX1; TFM; Dihydrotestosterone receptor; Nuclear Receptor Subfamily 3,Group C,Member 4; Testicular Feminization; Kennedy Disease
Assay Type: Sandwich
Sensitivity: 0.114 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P10275
Assay Type: Sandwich
Sensitivity: 0.114 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P10275
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