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ELK Biotechnology
SKU:ELK1804
Human MAPK14(Mitogen Activated Protein Kinase 14) ELISA Kit
Human MAPK14(Mitogen Activated Protein Kinase 14) ELISA Kit
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Alternative Names: CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; P38; Mitogen-activated protein kinase p38 alpha; Cytokine suppressive anti-inflammatory drug-binding protein
Assay Type: Sandwich
Sensitivity: 0.51 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAPK14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAPK14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAPK14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAPK14 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q16539
Assay Type: Sandwich
Sensitivity: 0.51 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAPK14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAPK14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAPK14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAPK14 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q16539
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