Human NT(Neurotensin) ELISA Kit

Alternative Names: NTS; NTS1; NmN-125; NN; Tail peptide Neuromedin N
Assay Type: Competitive Inhibition
Sensitivity: 14.2 pg/mL
standard: 3000 pg/mL
Detection range: 46.88-3000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Reproductive science;Neuro science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P30990