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ELK Biotechnology
SKU:ELK1704
Rat Bid(BH3 Interacting Domain Death Agonist) ELISA Kit
Rat Bid(BH3 Interacting Domain Death Agonist) ELISA Kit
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Alternative Names: p22 BID
Assay Type: Sandwich
Sensitivity: 0.063 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Apoptosis;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Bid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Bid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bid in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9JLT6
Assay Type: Sandwich
Sensitivity: 0.063 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Apoptosis;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Bid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Bid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bid in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9JLT6
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