Mouse NMB(Neuromedin B) ELISA Kit

Alternative Names: NM-B
Assay Type: Competitive Inhibition
Sensitivity: 4.87 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2.5h
Research Area: Signal transduction;Endocrinology;Neuro science;Gastroenterology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NMB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NMB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9CR53