Human HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit

Alternative Names: HSP60; GROEL; CPN60; HuCHA60; Heat Shock Protein 60; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein
Assay Type: Sandwich
Sensitivity: 0.59 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSPD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSPD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSPD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSPD1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P10809