Rat GIP(Gastric Inhibitory Polypeptide) ELISA Kit

Alternative Names: Incretin hormone;  Glucose Dependent Insulinotropic Peptide
Assay Type: Competitive Inhibition
Sensitivity: 25.8 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;Gastroenterology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GIP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GIP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q06145