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ELK Biotechnology
SKU:ELK1575
Human PYGM(Glycogen Phosphorylase, Muscle) ELISA Kit
Human PYGM(Glycogen Phosphorylase, Muscle) ELISA Kit
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Alternative Names: GPMM; Phosphorylase,Glycogen; McArdle Syndrome,Glycogen Storage Disease Type V; Myophosphorylase
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PYGM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PYGM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PYGM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PYGM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P11217
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PYGM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PYGM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PYGM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PYGM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P11217
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